Strategies to control the binding mode of de novo designed protein interactions
نویسندگان
چکیده
منابع مشابه
Binding of small molecules to cavity forming mutants of a de novo designed protein.
A central goal of protein design is to devise novel proteins for applications in biotechnology and medicine. Many applications, including those focused on sensing and catalysis will require proteins that recognize and bind to small molecules. Here, we show that stably folded α-helical proteins isolated from a binary patterned library of designed sequences can be mutated to produce binding sites...
متن کاملMetal-binding studies for a de novo designed calcium-binding protein.
To understand the key determinants in calcium-binding affinity, a calcium-binding site with pentagonal bipyramid geometry was designed into a non-calcium-binding protein, domain 1 of CD2. This metal-binding protein has five mutations with a net charge in the coordination sphere of -5 and is termed DEEEE. Fluorescence resonance energy transfer was used to determine the metal-binding affinity of ...
متن کاملTemplate-directed assembly of a de novo designed protein.
Many naturally occurring biomaterials are composed of laminated structures in which layers of beta-sheet proteins alternate with layers of inorganic mineral. These ordered laminates often have structural and mechanical properties that differ significantly from those of nonbiological materials. An important step in the construction of novel biomaterials is the creation of composites wherein a de...
متن کاملDe novo proteins from designed combinatorial libraries.
Combinatorial libraries of de novo amino acid sequences can provide a rich source of diversity for the discovery of novel proteins with interesting and important activities. Randomly generated sequences, however, rarely fold into well-ordered proteinlike structures. To enhance the quality of a library, features of rational design must be used to focus sequence diversity into those regions of se...
متن کاملDe novo designed cyclic-peptide heme complexes.
The structural characterization of de novo designed metalloproteins together with determination of chemical reactivity can provide a detailed understanding of the relationship between protein structure and functional properties. Toward this goal, we have prepared a series of cyclic peptides that bind to water-soluble metalloporphyrins (FeIII and CoIII). Neutral and positively charged histidine-...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2013
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2013.04.010